Melanotaenia fluviatilis adult ovary mRNA sequencing - SRA

SRX1007829: Melanotaenia fluviatilis adult ovary mRNA sequencing
1 ILLUMINA (Illumina HiSeq 2000) run: 30.5M spots, 6.1G bases, 3.5Gb downloads

Design: Total RNA was purified from approximately 15 mg ovary tissue using the RNeasy® Mini Kit (QIAGEN Pty. Ltd., Australia). Total RNA from whole brain was purified using the RNeasy® Lipid Tissue Kit (QIAGEN Pty. Ltd., Australia). On-column DNase treatments were included during purification. RNA integrity was confirmed using the Bioanalzyer 2100 (Agilent). Sequencing was undertaken by a service provider (Australian Genome Research Facility, Melbourne, Australia). Library preparation was performed using Illumina TruSeq reagents and sequencing was conducted using the HiSeq2000 platform. Briefly, RNA quality was determined using a Bioanalyzer 2100 (Agilent) before isolation of polyA+ mRNA, fragmentation, and construction of cDNA libraries using the Illumina TruSeq kit according to the standard protocol. 100-cycle paired-end sequencing was undertaken using two lanes of an Illumina HiSeq2000 instrument, with three libraries multiplexed per lane.

Submitted by: (CSIRO)

Study: Melanotaenia fluviatilis Assembly

PRJNA282059 • SRP057622 • All experiments • All runs

show Abstracthide Abstract

The primary goal of the study was to assemble a reference transcriptome of M.fluviatilis protein-coding sequences (mRNA) to facilitate future genome-wide expression studies (microarrays or RNA-Seq), focused gene expression studies (qRT-PCR) and protein functional characterization (e.g. in vitro receptor ligand sensitivity/specificity studies using reporter assays). M.fluviatilis is an emerging model organism used for ecotoxicological studies in the Australasia region.

Sample: M.fluviatilis ovary

SAMN03566737 • SRS920704 • All experiments • All runs

Organism: Melanotaenia fluviatilis

Library:

Name: FG

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 30.5M spots, 6.1G bases, 3.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR1994207	30,490,268	6.1G	3.5Gb	2015-07-01

ID:: 1462202

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